The CEM website (Co-evolution based enzyme mining) started ran from February 2022.
We provide more than 200,000 microbial genome data, which can be downloaded from the ncbi database, the microbial whole genome data according to the collated OGT data from Martin K. M. Engqvist et al., and a filamentous fungus database (https://pub.fungalgenomics.ca/). We will provide you with the information about the microbial genomes, from which the above mined candidate thermophilic enzymes are derived. We can achieve rapid mining of thermophilic enzymes in a short time of no more than ten minutes. Through this enzyme mining platform, we have discovered many thermophilic enzymes, all of which have great thermostability.
We provide three enzyme mining methods, which are based on the microbial optimum growth temperatures, the thermophilic fungus genomes, and the structural RNA GC content.
You need to first fill in your Job Name and email address, and then verify your email address before using the enzyme mining service.
Note: Your email must be real, otherwise it cannot be verified.
After verifying the email, users need to input the amino acid sequence of an enzyme in fasta
format In the search interface, the rapid mining of thermophilic enzymes can be
realized.
Note: Only an amino acid sequence in fasta format is allowed. Nucleotide sequences, special characters, and multiple amino acid sequences are not allowed.
Choose a method for thermophilic enzyme mining.
Note: Only one of the three mining methods can be selected for mining, but not at the same time.
Click Submit
. The information about mined candidate thermophilic enzymes will be sent to
users in the form of reports with .tsv
format, which can be downloaded directly from the
website.
The report can provide you with the information about the mined thermophilic enzymes.
The report of enzyme mining method based on microbial optimum growth temperature:
The report of enzyme mining method based on structural RNA GC content:
The report of enzyme mining method based on thermophilic fungus genomes: